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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Biologically active substances of the Gotu kola plant
 Šumberová, Michaela ; Nábělek, Jakub (referee) ; Zemanová, Jana (advisor)
The Bachelor thesis focuses on the preparation and characterization of the extract of Gotu kola (Centella asiatica). The theoretical part summarizes commonly analyzed groups of biologically active compounds of herbs and introduces potential applications of the extract in food industry. Moreover, an overview of possibly used extraction methods and analytical methods for the evaluation of biologically active compounds of the extract are presented. As for the experiment, the extract of the fresh Centella asiatica herb was prepared by maceration in ethanol and then analyzed by three chosen methods. The total phenolic content of the extract analyzed by the Folin-Ciocalteu test was 0.74 ± 0.08 mgGAE·g-1. As for the quantification of antioxidant activity, the TEAC test was performed and the antioxidant activity of the extract was 289 ± 81 µg·ml-1. The volatiles present in the extract were identified by gas-chromatography mass-spectrometry in combination with solid-phase micro-extraction. Sesquiterpenoid hydrocarbons were the most dominant group of the extract, in which -farnesene (33.28 %), -caryophyllene (25.01 %), and -humulene (20.91 %) were the major compounds.
Detailed record
Biologically active substances of the Gotu kola plant
 Šumberová, Michaela ; Nábělek, Jakub (referee) ; Zemanová, Jana (advisor)
The Bachelor thesis focuses on the preparation and characterization of the extract of Gotu kola (Centella asiatica). The theoretical part summarizes commonly analyzed groups of biologically active compounds of herbs and introduces potential applications of the extract in food industry. Moreover, an overview of possibly used extraction methods and analytical methods for the evaluation of biologically active compounds of the extract are presented. As for the experiment, the extract of the fresh Centella asiatica herb was prepared by maceration in ethanol and then analyzed by three chosen methods. The total phenolic content of the extract analyzed by the Folin-Ciocalteu test was 0.74 ± 0.08 mgGAE·g-1. As for the quantification of antioxidant activity, the TEAC test was performed and the antioxidant activity of the extract was 289 ± 81 µg·ml-1. The volatiles present in the extract were identified by gas-chromatography mass-spectrometry in combination with solid-phase micro-extraction. Sesquiterpenoid hydrocarbons were the most dominant group of the extract, in which -farnesene (33.28 %), -caryophyllene (25.01 %), and -humulene (20.91 %) were the major compounds.
Detailed record

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